O'Neill Family Pharmacy is an independent pharmacy at 317 E Douglas St in O'Neill, providing prescription fulfillment, over-the-counter medications, and personalized pharmacy services to the Holt County community.

The O'Neill Fire Department operates from a single station at 401 East Fremont Street with 36 members, including firefighters and EMTs, and three basic life support ambulances.

O'Neill Golf Club provides Golf at its best in north central Nebraska! The O'Neill Golf Club is located in the NW corner of O'Neill, just off Highway-281. The course was redesigned in 2002-03 and is one of the most challenging, yet playable 9-hole layouts for golfers of all ages.

O'Neill Headstart through CNCAP provides early childhood education and family support services for income-eligible children and families in the O'Neill and Holt County area.

Family owned bowling center in O'Neill, serving Holt County since 1985, with snacks, beverages and a full bar and grill.

O'Neill Lumber provides building materials, lumber, and hardware supplies for construction and home improvement projects in O'Neill, Nebraska and the Holt County area.

O'Neill Municipal Airport serves general aviation in O'Neill, Nebraska, providing runway facilities and aviation services for pilots and businesses in the Holt County area.

O'Neill Police Department is the municipal law enforcement agency serving the City of O'Neill, Nebraska, providing public safety and police services to the Holt County seat.

Full-service print shop in O'Neill. Business cards, letterheads, brochures, envelopes, forms, banners, flyers, color copies, and custom stamps.

O'Neill Public School Developing Eagles is an early childhood development program in O'Neill, Nebraska, providing preschool and developmental services for young children in the Holt County area.